A patatin-like protein with galactolipase activity is induced by drought stress in Vigna unguiculata leaves. (December 2000)

• Record Type: Journal Article
• Authors: Matos, A. R.; d'Arcy-Lameta, A.; França, M.; Zuily-Fodil, Y.; Pham-Thi, A. T.
• Is Part Of: Biochemical Society transactions. Volume 28:Number 6(2000)
• Page Start: 779
• Page End: 781
• Publication Date: 2000-12
• Subjects: cowpea -- lipolytic acylhydrolase; ABA, abscisic acid; Biochemistry -- Congresses; 572
• DOI: 10.1042/bst0280779
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : This paper reports the cloning of a cDNA ( Vupat 1 ) expressed in Vigna unguiculata leaves coding for a protein with 48% sequence homology to patatin, the major protein from potato tuber which has lipolytic acylhydrolase activity. Two cultivars differing in drought tolerance were ex-amined in Northern-blot analyses. Expression of Vupat 1 is stimulated by drought stress, especially in the drought-sensitive cultivar. Vupat 1 was expressed in the baculovirus system as a fusion protein secreted in the culture medium. The recombinant protein displays lipolytic activity towards monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerols.