Sequence-specific covalent labelling of DNA. (22nd March 2011)

• Record Type: Journal Article
• Authors: Gottfried, Anna; Weinhold, Elmar
• Is Part Of: Biochemical Society transactions. Volume 39:Number 2(2011)
• Page Start: 623
• Page End: 628
• Publication Date: 2011-03-22
• Subjects: DNA methyltransferase (DNA MTase) -- hairpin polyamide -- nicking endonuclease (NEase) -- S-adenosylmethionine analogue (AdoMet analogue) -- triple-helix-forming oligonu-cleotide; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST0390623
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Sequence-specific DNA modification is of significance for applications in bio- and nano-technology, medical diagnostics and fundamental life sciences research. Preferentially, labelling should be performed covalently, which avoids doubts about label dissociation from the DNA under various conditions. Several methods to label native DNA have been developed in the last two decades. Triple-helix-forming oligodeoxynucleotides and hairpin polyamides that bind DNA sequences specifically in the major and minor groove respectively were used as targeting devices for subsequent covalent labelling. In addition, enzyme-directed labelling approaches utilizing nicking endonucleases in combination with DNA polymerases or DNA methyltransferases have been employed. This review summarizes various techniques useful for functionalization of long native DNA.