Immunoperoxidase localization of type X collagen in chick tibiae. Issue 2 (February 1986)

• Record Type: Journal Article
• Authors: Kwan, Alvin P. L.; Freemont, Anthony J.; Grant, Michael E.
• Is Part Of: Bioscience reports. Volume 6:Issue 2(1986)
• Page Start: 155
• Page End: 162
• Publication Date: 1986-02
• Subjects: type X collagen -- tibiotarsus -- hypertrophic chondrocytes -- calcification; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01115001
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Type X collagen was prepared from medium of long-term cultures of embryonic chick tibiotarsal chondrocytes. Antibodies to type X collagen were raised and used in immunoperoxidase localization studies with embryonic and growing chick tibiotarsus. Strong anti-type X collagen reactivity was detected mainly in the region of hypertrophic chondrocytes, and to a lesser extent in the zone of calcified cartilage. No reactivity was detected in the proliferative zone nor the superficial layer of the cartilage growth plate. These results suggest that type X collagen may play a key role in matrix calcification during growth and development of the skeletal system.