Inductive activity of recombinant human growth and differentiation factor-5. (August 2000)

• Record Type: Journal Article
• Authors: Spiro, R. C.; Liu, L.-S.; Heidaran, M. A.; Thompson, A. Y.; Ng, C. K.; Pohl, J.; Poser, J. W.
• Is Part Of: Biochemical Society transactions. Volume 28:Number 4(2000)
• Page Start: 362
• Page End: 368
• Publication Date: 2000-08
• Subjects: bone graft matrix -- bone morphogenetic protein -- segmental bone defect -- spine fusion; ECM, extracellular matrix -- GDF-5, growth and differentiation factor-5 -- rhGDF-5, recombinant human GDF-5 -- BMP, bone morphogenetic protein -- QHMA, quantitative histomorphometric analysis -- PNB, percentage of new bone -- PRD, percentage of residual defect -- CT, computerized tomographic -- L, lumbar; Biochemistry -- Congresses; 572
• DOI: 10.1042/bst0280362
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Growth and differentiation factor-5 (GDF-5) is a divergent member of the transforming growth factor-β/bone morphogenetic protein (BMP) superfamily that is required for proper skeletal patterning and development in the vertebrate limb. Based on the homology of GDF-5 with other bone-inducing BMP family members, the inductive activity of a recombinant form of human GDF-5 (rhGDF-5) was evaluated in a series of in vitro assays and in vivo bone-formation models. The in vitro response to rhGDF-5 resulted in the formation of chondrogenic nodules in fetal rat calvarial cells cultured in the context of collagen or collagen/hyaluronate extracellular matrices. Matrices loaded with rhGDF-5 induced ectopic cartilaginous and osseous tissue when implanted in subcutaneous or intramuscular sites. In non-human primate long-bone-defect and spinal-fusion models, rhGDF-5 combined with a mineralized collagen matrix induced bone formation in a manner equivalent to autogenous bone. These results highlight the unique potential of rhGDF-5 in a wide variety of orthopaedic applications.