The basal permeability to water of human red blood cells evaluated by a nuclear magnetic resonance technique. Issue 1 (February 1990)

• Record Type: Journal Article
• Authors: Benga, Gheorghe; Pop, Victor Ioan; Popescu, Octavian; Borza, Victoria
• Is Part Of: Bioscience reports. Volume 10:Issue 1(1990)
• Page Start: 31
• Page End: 36
• Publication Date: 1990-02
• Subjects: erythrocyte -- nuclear magnetic resonance -- water permeability; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01116848
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : The characteristics of water diffusional permeability (P) of human red blood cells were studied on isolated erythrocytes by a doping nuclear magnetic resonance technique. In order to estimate the basal permeability the maximal inhibition of water diffusion was induced by exposure of red blood cells to p-chloromercuribenzene sulfonate (PCMBS) under various conditions (concentration, duration, temperature). The lowest values of P were around 0.7×10 −3 cm s −1 at 10°C, 1.2×10 −3 cm s −1 at 15°C, 1.4×10 −3 cm s −1 at 20°C, 1.8×10 −3 cm s −1 at 25°C, 2.1×10 −3 cm s −1 at 30°C and 3.5×10 −3 cm s −1 at 37°C. The mean value of the activation energy of water diffusion (Ea, d ) was 25 kJ/mol for control and 43.7 kJ/mol for PCMBS-inhibited erythrocytes. The values of P and Ea, d obtained after induction of maximal inhibition of water diffusion by PCMBS can be taken as references for the basal permeability to water of the human red blood cell membrane.