Immunoprecipitation of a pertussis toxin substrate of the Go family from rat islets of Langerhans. Issue 2 (April 1992)

• Record Type: Journal Article
• Authors: Berrow, Nicholas S.; Hurst, Roger D.; Chan, Susan L. F.; Morgan, Noel G.
• Is Part Of: Bioscience reports. Volume 12:Issue 2(1992)
• Page Start: 95
• Page End: 100
• Publication Date: 1992-04
• Subjects: Insulin secretion -- pancreatic B-cell -- G-protein -- adenylate cyclase; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF02351213
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Rat islets express a pertussis toxin sensitive G-protein involved in receptor-mediated inhibition of insulin secretion. This has been assumed previously to represent "Gi " which couples inhibitory receptors to adenylate cyclase. Incubation of islet G-proteins with 32 P-NAD and pertussis toxin resulted in the labelling of a band of molecular weight 40, 000. This band was very broad and did not allow resolution of individual components. Incubation of the radiolabelled proteins with an anti-Go antiserum resulted in specific immunoprecipitation of a 32 P-labelled band. These results demonstrate that the complement of pertussis toxin sensitive G-proteins in rat islets includes Go .