Inverted-repeat DMA: a new gene-silencing tool for seed lipid modification. (December 2000)

• Record Type: Journal Article
• Authors: Singh, S.; Green, A.; Stoutjesdijk, P.; Liu, Q.
• Is Part Of: Biochemical Society transactions. Volume 28:Number 6(2000)
• Page Start: 925
• Page End: 927
• Publication Date: 2000-12
• Subjects: Arabidopsis -- Δ12-desaturase -- dsRNA; PTGS, post-transcriptional gene silencing -- ODP, oleic desaturation proportion; Biochemistry -- Congresses; 572
• DOI: 10.1042/bst0280925
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Post-transcriptional gene silencing (PTGS) has been successfully used to modify seed lipids in oilseed crops like soybean, canola and sunflower. Conventionally, PTGS has been induced by transforming the plants with either antisense or co-suppression constructs targeted against key seed lipid biosynthesis genes. A major drawback of this approach has been the recovery of only a modest proportion of silenced individuals from large populations of transgenic plants. In this report we show that inverted-repeat DNA constructs containing an intron encoding RNA with a hairpin structure can induce PTGS with very high frequency.