Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis. Issue 5 (May 1986)

• Record Type: Journal Article
• Authors: Kitamoto, Tetsuyuki; Hikita, Kohtaro; Tashima, Takatoshi; Tateishi, Jun; Sato, Yuji
• Is Part Of: Bioscience reports. Volume 6:Issue 5(1986)
• Page Start: 459
• Page End: 465
• Publication Date: 1986-05
• Subjects: SAF -- amyloid fibril protein -- Alzheimer's disease -- systemic amyloidosis; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01116137
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.