Mass spectrometry: Future opportunities for profiling and imaging steroids and steroid metabolites. (December 2020)

• Record Type: Journal Article
• Authors: Andrew, Ruth; Homer, Natalie Z.M.
• Is Part Of: Current opinion in endocrine and metabolic research. Volume 15(2020)
• Page Start: 71
• Page End: 78
• Publication Date: 2020-12
• Subjects: Steroid -- Estrogen -- Mass spectrometry -- Chromatography -- Imaging -- Extraction; CCS collision cross-section -- DESI desorption electrospray ionisation -- GC Gas Chromatography -- HILIC hydrophilic interaction liquid chromatography -- HPLC high performance liquid chromatography -- IMS ion mobility separation -- LC Liquid chromatography -- LESA liquid extraction surface analysis -- MALDI Matrix assisted laser desorption ionisation -- MIP Molecular Imprinted polymers -- MS Mass spectrometry -- SLE supported liquid extraction -- SPE solid phase extraction -- UHPLC ultra-HPLC; Endocrine glands -- Diseases -- Periodicals; Metabolism -- Disorders -- Periodicals; Endocrine System Diseases; Metabolic Diseases; Endocrine glands -- Diseases; Metabolism -- Disorders; Periodical; Electronic journals; Periodicals; Electronic journals; 616.4
• DOI: 10.1016/j.coemr.2020.11.005
• Languages: English
• ISSNs: 2451-9650

Abstract

Abstract: Steroid hormone profiling has historically underpinned advances in endocrine investigation and research, crucially dependent on selective and sensitive hormone assays. Mining the "steroidome" by mass spectrometry (MS) provides greater specificity than immunoassays. Building on a 50 year legacy, gas and liquid chromatography-MS continue to evolve (e.g. sequential derivatisation, mobile phase modifiers). Exciting new technology (e.g. imaging, ion mobility, supercritical fluid), sample preparation (microextraction, molecular imprinted polymers) are advancing the field. Automated analysis of wider profiles of steroids is within reach, in smaller and more varied sample types, including molecular mapping of tissue sections. These new analytical dimensions require rigorous validation and advanced statistics. This review highlights that MS continues to open new windows of biochemical understanding in endocrinology. Graphical abstract: Image 1 Highlights: Mass spectrometry offers greater specificity than immunoassays for mining the steroidome. Sample preparation can be automated and refined by micro- and bespoke fractionation, with spatial sampling now possible. Gas and liquid chromatography linked to mass spectrometry are evolving via technology, column chemistry and derivatization. Orthogonal separation by ion mobility and supercritical fluid chromatography are enabling analysis of complex matrices. Multi-profiling is possible by aligning quantitative steroidomics with advanced statistical approaches and machine learning.