Comparative genomic analyses of IncpA1763‐KPC plasmids. (8th February 2021)

• Record Type: Journal Article
• Authors: Chen, Hongchao; Zhan, Zhe; Jiang, Xiaoyuan; Qing, Yang; Yin, Zhe; Mei, Lingling; Zhou, Dongsheng; Ni, Bin; Zhang, Yanjun
• Is Part Of: Journal of basic microbiology. Volume 61:issue 3(2021)
• Page Start: 219
• Page End: 229
• Publication Date: 2021-02-08
• Subjects: antibiotic resistance -- IncpA1763‐KPC plasmids -- Klebsiella pneumonia -- mobile elements; Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.202000668
• Languages: English
• ISSNs: 0233-111X

Abstract

Abstract: Multi‐replicon plasmids harboring the IncpA1763‐KPC replicon together with other replicons are being increasingly reported among Enterobacteriaceae species. However, plasmids with single IncpA1763‐KPC replicons are poorly studied as a different incompatibility (Inc) group, despite their rise in appearance in some strains. IncpA1763‐KPC plasmids, pA1763‐KPC, and p427113‐2, from two clinical Klebsiella pneumoniae isolates were fully sequenced by high‐throughput genome sequencing. Linear structural comparisons of IncpA1763‐KPC backbone region were made between these two plasmids and six arbitrarily selected representative IncpA1763‐KPC plasmids sequenced previously. A further detailed genomic comparison was carried out between plasmids pA1763‐KPC, p427113‐2, and pFB2.2, which show high homology across the backbone sequence to one another. Among all sequenced IncpA1763‐KPC plasmids considered in this study, plasmids pA1763‐KPC and p427113‐2 showed the most complete IncpA1763‐KPC backbones. These were composed of the IncpA1763‐KPC replicon ( repA IncpA1763‐KPC and its iterons), the 5.6‐kb IncpA1763‐KPC ‐type maintenance region, the 27.7‐kb IncFIIK ‐type maintenance region, and the 36.6‐kb IncFIIK ‐type conjugal transfer regions. Compared with pA1763‐KPC or p427113‐2, the backbone regions of the other analyzed IncpA1763‐KPC plasmids had gradually undergone different deletions or truncations, but shared small and core IncpA1763‐KPC backbones including the IncpA1763‐KPC replicon, IncpA1763‐KPC ‐type maintenance region, and residual IncFIIK ‐type maintenance region. Accessory modules integrated into IncpA1763‐KPC backbones included the multidrug‐resistant module bla KPC‐2 region in pA1763‐KPC, the metal‐resistance modules ars region together with ncr region in pFB2.2 and sil in pKPN‐9a0d, the IS Kpn14 ‐to‐IS 26 region in p427113‐2, and other non‐resistance region in the respective plasmids. This detailed comparative genomics analysis of IncpA1763‐KPC plasmids provides a deep insight into their diversification and evolution.