Cytoskeleton induced the changes of microvilli and mechanical properties in living cells by atomic force microscopy. Issue 5 (10th November 2020)

• Record Type: Journal Article
• Authors: Liu, Xueyan; Wei, Yuhui; Li, Wei; Li, Bin; Liu, Lin
• Is Part Of: Journal of cellular physiology. Volume 236:Issue 5(2021)
• Page Start: 3725
• Page End: 3733
• Publication Date: 2020-11-10
• Subjects: atomic force microscopy -- mechanical properties -- microfilaments -- microtubules -- microvilli; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.30110
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: The cytoskeleton acts as a scaffold for membrane protrusion, such as microvilli. However, the relationship between the characteristics of microvilli and cytoskeleton remains poorly understood under the physiological state. To investigate the role of the cytoskeleton in regulating microvilli and cellular mechanical properties, atomic force microscopy (AFM) was used to detect the dynamic characteristics of microvillus morphology and elastic modulus of living HeLa cells. First, HeLa and MCF‐7 cell lines were stained with Fluor‐488‐phalloidin and microtubules antibody. Then, the microvilli morphology was analyzed by high‐resolution images of AFM in situ. Furthermore, changes in elastic modulus were investigated by the force curve of AFM. Fluorescence microscopy and AFM results revealed that destroyed microfilaments led to a smaller microvilli size, whereas the increase in the aggregation and number of microfilaments led to a larger microvilli size. The destruction and aggregation of microfilaments remarkably affected the mechanical properties of HeLa cells. Microtubule‐related drugs induced the change of microtubule, but we failed to note significant differences in microvilli morphology and mechanical properties of cells. In summary, our results unraveled the relationship between microfilaments and the structure of microvilli and Young's modulus in living HeLa cells, which would contribute to the further understanding of the physiological function of the cytoskeleton in vivo. Abstract : In this study, atomic force microscopy (AFM) was used to detect the dynamic characteristics of microvillus morphology and elastic modulus of living HeLa cells. Our results directly unraveled the relationship between microfilaments and the structure of microvilli and Young′s modulus in living HeLa cells, which would contribute to the further understanding of the physiological function of the cytoskeleton in vivo.