Effective Separation of Human Milk Glycosides using Carbon Dioxide Supercritical Fluid Chromatography. Issue 5 (25th January 2021)

• Record Type: Journal Article
• Authors: Liou, Shih‐Wei; Fang, Jia‐Lin; Lin, Hung‐Wei; Tsai, Teng‐Wei; Huang, Hsin‐Hui; Liang, Chin‐Yu; Yang, Cheng‐Ruel; Wei, Guor‐Tzo; Yu, Ching‐Ching
• Is Part Of: Chemistry, an Asian journal. Volume 16:Issue 5(2021)
• Page Start: 492
• Page End: 497
• Publication Date: 2021-01-25
• Subjects: Supercritical fluid chromatography -- oligosaccharides -- carbohydrates -- separations -- glycosides; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.202001404
• Languages: English
• ISSNs: 1861-4728

Abstract

Abstract: Carbohydrate purification remains problematic due to the intrinsic diversity of structural isomers present in nature. Although liquid chromatography‐based techniques are suitable for analyzing or preparing most glycan structures acquired either from natural sources or through chemical or enzymatic synthesis, the separation of regioisomers or linkage isomers with a clear resolution remains challenging. Herein, a carbon dioxide supercritical fluid chromatography (SFC) method was devised to resolve 18 human milk glycosides: oligomers (disaccharides to hexasaccharides), fucosylated regioisomers (lacto‐ N ‐fucopentaose I, III, and V; lacto‐ N ‐neofucopentaose V; lacto‐ N ‐difucohexaose III; blood group H1 antigen; and TF‐LNnT), and connectivity isomers (lacto‐ N ‐tetraose/lacto‐ N ‐neotetraose and para ‐lacto‐ N ‐hexaose/ para ‐lacto‐ N ‐neohexaose/type‐1 hexasaccharide). The analysis of these glycosides represents a major limitation associated with conventional carbohydrate analysis. The unprecedented resolution achieved by the SFC method indicates the suitability of this key technology for revealing complex human milk glycomes. Abstract : We report a green chromatography approach, a carbon dioxide supercritical fluid chromatography (SFC)‐based technique, and address the major concern of human milk glycan isomer separation through SFC which provided favorable resolutions of regioisomers and linkage isomers in a short retention time.