A simple method to construct a low-cost immunosensor based on a dithiol-functionalized polydopamine platform. (15th February 2021)

• Record Type: Journal Article
• Authors: Liu, Bo; Yang, Luanying; Wang, Gang; He, Sha; Wang, Xiaobo; Ye, Ling
• Is Part Of: New journal of chemistry. Volume 45:Number 8(2021)
• Page Start: 3940
• Page End: 3946
• Publication Date: 2021-02-15
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d0nj06241c
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A simple and low-cost electrochemical CEA immunosensor was investigated via the self-polymerization of dopamine and a dithiol compound spacer for the covalent immobilization of antibodies. The designed CEA immunosensor exhibited a linear response and a low detection limit. Abstract : In this study, a simple and low-cost electrochemical immunosensor for the detection of tumor markers (carcinoembryonic antigen, CEA, as a model) was investigated. The self-polymerization of dopamine was used to provide a secondary reaction platform, and a more stable dithiol compound (thioctic acid) as a spacer was fixed on the platform via the Michael's addition reaction. A dithiol-functionalized platform was used for the covalent immobilization of antibodies, and the structure showed significant changes in the atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) images. The electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) measurements were performed to test the performance of a double-antibody sandwich structure. The designed CEA immunosensor exhibited a linear response over a concentration range of 0.05–80 ng mL −1 with a low detection limit of 1.21 pg mL −1, and the results demonstrated a potential application for clinical diagnosis.