PEI-assisted boronate affinity magnetic nanoparticle-based SELEX for efficient in vitro evolution of saponin-binding aptamers. Issue 15 (25th February 2021)

• Record Type: Journal Article
• Authors: Zhang, Hui; Li, Xue; Huang, Ailan; Yan, Zhifeng; Chen, Yang; Bie, Zijun
• Is Part Of: RSC advances. Volume 11:Issue 15(2021)
• Page Start: 8775
• Page End: 8781
• Publication Date: 2021-02-25
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra00889g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Branched polyethyleneimine assisted boronate affinity magnetic nanoparticle-based aptamer evolution platform. Abstract : Saponin is a large family of important natural products with various pharmacological activities. Selective enrichment of saponin from complex biological samples is a key step for analysis of saponin. Despite that aptamers have been widely used for selective enrichment, aptamers that can specifically recognize saponins have never been reported. In this study, a facile and efficient SELEX approach was developed for in vitro evolution of saponin-binding aptamers, using PEI-assisted boronate affinity magnetic nanoparticles (p-BA-MNPs) that exhibit highly favorable binding properties as a general affinity platform. As a proof of the principle, ginsenoside Re and Rb1 were employed as two target saponins. Two aptamers towards each target saponin, with dissociation constant at the 10 −5 M level, were selected within 6 rounds. An affinity magnetic nanoparticle was constructed by using the selected aptamer as a affinity ligand. The resulting material allowed for the quantitative analysis of ginsenoside Re in real samples with high reliability. The p-BA-MNPs based SELEX is straightforward and generally applicable for a wide range of target saponins, providing a promising aptamer evolution approach for aptamer-based research and pharmaceutical analysis.