A high-performance microfluidic detection platform to conduct a novel multiple-biomarker panel for ovarian cancer screening. Issue 14 (19th February 2021)

• Record Type: Journal Article
• Authors: Wu, Yu; Wang, Chunhua; Wang, Pan; Wang, Chao; Zhang, Yu; Han, Lin
• Is Part Of: RSC advances. Volume 11:Issue 14(2021)
• Page Start: 8124
• Page End: 8133
• Publication Date: 2021-02-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra10200h
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A high-performance microfluidic detection platform is developed to conduct a novel panel of multiple biomarkers for the early detection of ovarian carcinoma, which is promising for the early detection of ovarian cancer. Abstract : Ovarian cancer is an important leading cause of cancer-related deaths among females, and a single biomarker does not have the sensitivity and specificity required for an effective ovarian cancer screening. Herein, we investigate a high-performance microfluidic detection platform to conduct a novel panel of multiple biomarkers for the early detection of ovarian carcinoma, which include CA125, HE4, OPN, MSLN, Hsp70, CA153, AFP, IL-6, and IL-8 using a microfluidic chip. High-throughput microfluidic chips and graphene oxide-assembled substrate are used to microprint repeatable capture antibody arrays and conduct multiple biomarkers in microscale volume samples. The proposed microfluidic platform achieves an ultralow detection limit of ∼1 pg mL −1 and 0.01 U mL −1 with excellent detection selectivity and a short detection time of 30 min. The analysis of serum biomarkers in 18 ovarian cancer patients and 4 healthy persons indicates a clear subgroup sorting between the high-grade serous ovarian carcinoma, borderline, and benign tumor patients, and healthy persons. The proposed detection platform and the biomarker panel are promising to conduct an early detection of ovarian cancer.