Bulges in left-handed G-quadruplexes. Issue 3 (27th January 2021)

• Record Type: Journal Article
• Authors: Das, Poulomi; Ngo, Khac Huy; Winnerdy, Fernaldo Richtia; Maity, Arijit; Bakalar, Blaž; Mechulam, Yves; Schmitt, Emmanuelle; Phan, Anh Tuân
• Is Part Of: Nucleic acids research. Volume 49:Issue 3(2021)
• Page Start: 1724
• Page End: 1736
• Publication Date: 2021-01-27
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkaa1259
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.