The lipopolysaccharide model for the experimental induction of transient lameness and synovitis in Standardbred horses. (April 2021)

• Record Type: Journal Article
• Authors: Van de Water, E.; Oosterlinck, M.; Korthagen, N.M.; Duchateau, L.; Dumoulin, M.; van Weeren, P.R.; Olijve, J.; van Doorn, D.A.; Pille, F.
• Is Part Of: Veterinary journal. Volume 270(2021)
• Publication Date: 2021-04
• Subjects: Arthritis -- Joint disease -- Lameness -- Lipopolysaccharide -- Synovitis; Veterinary medicine -- Periodicals; 636
• DOI: 10.1016/j.tvjl.2021.105626
• Languages: English
• ISSNs: 1090-0233

Abstract

Highlights: Lipopolysaccharide (LPS; 10 endotoxin units) in the middle carpal joint induced lameness. Peak lameness was observed at 4 h post-injection, returning to baseline within 48 h. Lameness was prevented by meloxicam daily for 4 days prior to LPS injection. Pre-treatment with meloxicam reduced prostaglandin(PG)E2 in synovial fluid. LPS for injection requires uniform preparation and handling. Abstract: An established lipopolysaccharide (LPS) model previously described in Warmbloods, was inconsistent in Standardbred horses, where lameness was not detected despite the presence of synovitis. The present study aimed to determine the dose of LPS from E. coli O55:B5 required to induce mild to moderate lameness following middle carpal joint injection in Standardbred horses and to quantitate the induced lameness over time, with and without anti-inflammatory pre-treatment. In a baseline trial, eight healthy, clinically sound Standardbred horses were used in a rule-based dose-escalation design trial, starting at a dose of 10 endotoxin units (EU). Lameness at trot was evaluated visually and quantitatively (using an inertial-sensor system and pressure plate analysis). Synovial fluid aspirates were analysed for total nucleated cell counts, total protein and prostaglandin E2 (PGE2 ). Following 2 months wash-out, the effective LPS-dose determined in the baseline trial was used to evaluate the effect of anti-inflammatory treatment. A mixed model for repeated measures with horse as random effect was used for analysis. After injection of 10 EU LPS, the desired degree of lameness was observed in the baseline trial, with maximal lameness at post-injection hour (PIH) 4, followed by a rapid decline and return to baseline by PIH 48. No lameness was observed following pre-treatment with meloxicam. In synovial fluid, PGE2 was significantly higher at PIH 8 and PIH 24 in the baseline trial compared with following meloxicam pre-treatment. In conclusion, injection of the middle carpal joint with 10 EU LPS consistently induces a transient lameness and synovitis in Standardbred horses.