Cervicovaginal microbiota dysbiosis correlates with HPV persistent infection. (March 2021)

• Record Type: Journal Article
• Authors: Qingqing, Bi; Jie, Zhu; Songben, Qu; Juan, Chen; Lei, Zhang; Mu, Xiaofeng
• Is Part Of: Microbial pathogenesis. Volume 152(2021)
• Publication Date: 2021-03
• Subjects: HPV persistent Infection -- Cervicovaginal microbiota -- 16S rDNA sequencing -- Host immunosuppression; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104617
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: HPV persistent infection is a main event leading to the development of cervical intraepithelial neoplasia and cervical cancer. Earlier to distinguish HPV persistent and transient infection is meaningful but the methods are limited. This study used 16S rDNA sequencing to determine the cervicovaginal microbiota of HPV persistent infection, transient infection and health women. Sequences analysis was performed and according to subsequent statistical analysis, the structure of cervicovaginal microbiota of healthy and transient infection individuals is relatively single, Firmicutes occupy the main composition. However, that of the HPV persistent infection presented a complicated trend and the abundance of Proteobacteria, Actinobacteria, Bacteroidetes and Fusobacteria was higher. The significance p-values of the average species abundance of Firmicutes, Proteobacteria and Bacteroides between HPV persistent and transient infection groups were 0.003, 0.018 and 0.005, respectively. The study also found 36 biomarkers of cervicovaginal microbiota dysbiosis for LDA score>4 among different groups. At genus level, Prevotella, Sphingomonas and Anaerococcus correlated with HPV persistent infection. At species level, Lactobacillus iners correlated with HPV transient infection. Besides, local immune microenvironment was changed with cervicovaginal microbiota dysbiosis. Interleukin-6 and TNF-α were significantly upregulated in cervical secretions from HPV persistent infection compared with those from transient infection and healthy women. Peripheral blood Regulatory T cells and myeloid-derived suppressor cells in patients with HPV persistent infection were also significantly increased. In conclusion, this study identified cervicovaginal microbiota dysbiosis closely related to HPV persistent infection, which provided a new idea and method for the prevention of cervical cancer. Highlights: The abundance of anaerobe was higher HPV in persistent infection patients. Prevotella, Sphingomonas and Anaerococcus correlated with HPV persistent infection. Immunosuppressive cells and cytokines were upregulated in HPV persistent infection patients. Thirty-six biomarkers for LDA score>4 among different groups were found.