Carbon nitride nanoparticles as ultrasensitive fluorescent probes for the detection of α-glucosidase activity and inhibitor screening. Issue 3 (9th December 2020)

• Record Type: Journal Article
• Authors: Guo, Feng-Na; Wang, Yi-Ting; Wu, Na; Feng, Li-Xia; Zhang, Hui-Chao; Yang, Ting; Wang, Jian-Hua
• Is Part Of: Analyst. Volume 146:Issue 3(2021)
• Page Start: 1016
• Page End: 1022
• Publication Date: 2020-12-09
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an02079f
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A novel strategy for the detection of α-glucosidase activity and inhibitor screening was developed based on fluorescent carbon nitride nanoparticles. Abstract : In recent years, α-glucosidase inhibitors (AGIs) have played a significant role in the treatment of type II diabetes (T2D), so it is necessary to develop a reliable and sensitive method to find new AGIs. Herein, we establish a novel method based on fluorescent carbon nitride nanoparticles (CNNPs) for the sensitive detection of the activity of α-glucosidase (α-glu) and the screening of its inhibitors. A CNNP-based fluorescent probe is synthesized from green raw materials, urea and lysine, by a one-pot method. In the presence of α-glu, the substrate 4-nitrophenyl-α-d -glucopyranoside (pNPG) is hydrolyzed to generate 4-nitrophenol (pNP), leading to the fluorescence (FL) quenching of CNNPs due to the inner filter effect (IFE). On the other hand, the activity of α-glu is inhibited after the addition of AGIs, which turns on the FL of CNNPs. In this way, the detection of α-glu activity and the screening of AGIs are achieved. The linear range is 1.25–10.00 U L −1 with a limit of detection as low as 0.17 U L −1 and the IC50 values of two typical inhibitors (gallic acid and acarbose) are 813 μM and 465 μM, respectively. The CNNP probe is further applied for the determination of α-glu activity in human serum samples with satisfactory results.