Impact of membrane protein-lipid interactions on formation of bilayer lipid membranes on SAM-modified gold electrode. (20th March 2021)

• Record Type: Journal Article
• Authors: Kato, Masaru; Masuda, Yuya; Yoshida, Narumi; Tosha, Takehiko; Shiro, Yoshitsugu; Yagi, Ichizo
• Is Part Of: Electrochimica acta. Volume 373(2021)
• Publication Date: 2021-03-20
• Subjects: Membrane protein -- Surface-enhanced infrared absorption spectroscopy -- Nitric oxide reductase -- Protein-tethered bilayer lipid membrane -- Protein film electrochemistry; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2021.137888
• Languages: English
• ISSNs: 0013-4686

Abstract

Highlights: A protein-tethered bilayer lipid membrane (ptBLM) was assembled on SAM/Au. The ptBLM assembly was monitored by time-resolved SEIRA spectroscopy. The lipid bilayer formation kinetics was influenced by protein–lipid interactions. Protein–lipid interactions improved the electrocatalytic stability of the cNOR. Abstract: In protein film electrochemistry for redox-active membrane proteins, the reconstitution of protein-tethered bilayer lipid membranes (ptBLMs) can maximize their functionality and stability at electrolyte/electrode interfaces. To understand the impact of protein–lipid interactions on the ptBLM formation, we tracked the reconstitution process of a lipid bilayer in the presence or the absence of a transmembrane enzyme of cytochrome c -dependent nitric oxide reductase (cNOR) on a mixed self-assembled monolayer (SAM)/Au electrode. Time-resolved surface-enhanced infrared absorption (SEIRA) spectroscopy and electrochemical measurements revealed that the protein–lipid interaction affected the formation kinetics for the phospholipid bilayer and the electrocatalytic stability of the cNOR-modified electrode but not the fluidity of the phospholipid bilayer. Graphical abstract: Image, graphical abstract