Efficacy of Rg1-Oil Adjuvant on Inducing Immune Responses against Bordetella bronchiseptica in Rabbits. (29th January 2021)

• Record Type: Journal Article
• Authors: Chenwen, Xiao; Quanan, Ji; Yee, Huang; Yan, Liu; Jiaoyu, Wang; Qiang, Wei; Litao, Qiao; Li, Nan; Guolian, Bao
• Other Names: Niedźwiedzka-Rystwej Paulina Academic Editor.
• Is Part Of: Journal of immunology research. Volume 2021(2021)
• Publication Date: 2021-01-29
• Subjects: Immunology -- Periodicals; Immunology -- Research -- Periodicals; 616.07905
• DOI: 10.1155/2021/8835919
• Languages: English
• ISSNs: 2314-8861

Abstract

Abstract : Bordetella bronchiseptica ( B . bronchiseptica ) is an obligately aerobic, oxidase- and catalase-positive, nonfermentative Gram-negative coccobacillus. This study is aimed at examining the immune effects of Rg1, Rg1 plus oil, and other common adjuvants on inactivated B . bronchiseptica vaccine in rabbits. The mechanism underlying the adjuvant effect of Rg1 plus oil on the vaccine was also explored. Rg1 (100 μ g) plus oil significantly improved the immune effect of B . bronchiseptica vaccine at both the humoral and cellular levels. Rg1-oil adjuvant increased the levels of IL-2 and IL-4 in rabbits after immunization. Rg1 (100 μ g) plus oil also significantly increased TLR2 expression and downregulated NF- κ B in splenocytes. Rg1-oil adjuvant may increase the levels of IL-2 and IL-4 via upregulating TLR2, thereby enhancing the immune effect of B . bronchiseptica vaccine. In conclusion, Rg1 plus oil could be used as a potential vaccine adjuvant for rabbit B . bronchiseptica vaccine.