Utilization of Response Surface Methodology in Optimization of Polysaccharides Extraction from Vietnamese Red Ganoderma lucidum by Ultrasound-Assisted Enzymatic Method and Examination of Bioactivities of the Extract. (11th February 2021)

• Record Type: Journal Article
• Authors: Do, Dat Tran; Lam, Dang Hoang; Nguyen, Tai; Phuong Mai, Tran Thi; Phan, Le Thao My; Vuong, Hoai Thanh; Nguyen, Duc Viet; Linh, Ngo Thi Thuy; Hoang, Minh Nam; Mai, Thanh Phong; Nguyen, Huu Hieu
• Other Names: Sarkar Nirmal Kumar Academic Editor.
• Is Part Of: TheScientificWorldjournal. Volume 2021(2021)
• Publication Date: 2021-02-11
• Subjects: Science -- Periodicals; Technology -- Periodicals; Medicine -- Periodicals; 505
• DOI: 10.1155/2021/7594092
• Languages: English
• ISSNs: 2356-6140

Abstract

Abstract : Red Ganoderma lucidum (G. lucidum) is a popular medicinal herb commonly used in Vietnamese traditional remedies due to its potential value for health. In this study, polysaccharides were extracted from G. lucidum using ultrasound-assisted enzymatic extraction method. The response surface methodology and Box–Behnken design were employed to investigate the effects of pH, extraction temperature, extraction time, and ultrasonic power on the content of polysaccharides. Based on ultraviolet-visible spectroscopy analysis, the highest content of polysaccharides in the extract was 32.08 mg/g under optimum experimental parameters including enzyme concentration of 3%, pH of 5.5, extraction temperature of 45°C, extraction time of 30 min, and ultrasonic power of 480 W. The Fourier-transform infrared spectroscopy was also used to identify the functional groups in the extracts. The molecular weights of polysaccharides were determined by gel permeation chromatography. The obtained extract was then evaluated for anticancer activities by using (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay, showing the anticancer activities with the half-maximal inhibitory concentration value of more than 512 μ g/mL. This result suggested that UAEE could be considered as an appropriate and effective extraction method for bioactive crude polysaccharides from G. lucidum.