The validation of the role of several genes related to Bombyx mori nucleopolyhedrovirus infection in vivo. Issue 2 (8th January 2021)

• Record Type: Journal Article
• Authors: Wang, Xue‐yang; Zhao, Chun‐xiao; Wang, Xin; Zhao, Zi‐qin; Su, Zhi‐hao; Xu, Ping‐zhen; Li, Mu‐wang; Wu, Yang‐chun
• Is Part Of: Archives of insect biochemistry and physiology. Volume 106:Issue 2(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-01-08
• Subjects: antiviral infection -- BmNPV -- Bombyx mori -- functional verification; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21762
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: Bombyx mori nucleopolyhedrovirus (BmNPV) is one of primary silkworm pathogens and causes a serious damage of cocoon losses every year. Recent years, many works have been done to clarify the silkworm anti‐BmNPV mechanism, and a significant progress has been made in screening and studying of genes and proteins related to BmNPV infection, but several of them lacked the proofs in vivo. In this study, to further validate the function of seven newly reported genes in vivo, including BmAtlatin‐n, Bmferritin‐heavy chain ( BmFerHCH ), Bmthymosin ( BmTHY ), Bmseroin1, Bmseroin2, Bmnuclear hormone receptors 96 ( BmNHR96 ), and BmE3 ubiquitin‐protein ligase SINA‐like 10 ( BmSINAL10 ), the response of them in the midgut, fat body, and hemolymph of differentially resistant strains (resistant strain YeA and susceptible strain YeB) at 48 h following BmNPV infection were analyzed. The results showed that the relative stable or upregulated expression level of BmAtlatin‐n, BmTHY, Bmseroin1, and Bmseroin2 in YeA resistant strain following BmNPV infection further indicated their antiviral role in vivo, compared with susceptible YeB strain. Moreover, the significant downregulation of BmFerHCH, BmNHR96, and BmSINAL10 in both strains following BmNPV infection revealed their role in benefiting virus infection, as well as the upregulation of BmFerHCH in YeB midgut and BmSINAL10 in YeB hemolymph. These data could be used to complementary the proofs of the function of these genes in response to BmNPV infection. Abstract : In this study, the function of seven newly reported genes, including BmAtlatin‐n, BmFerHCH, BmTHY, Bmseroin1, Bmseroin2, BmNHR96, and BmSINAL10, were validated to be related with BmNPV infection in vivo. Breifly, the expression patterns of them in the midgut, fat body, and hemolymph of different resistant strains (resistant strain YeA and susceptible strain YeB) at 48 h following BmNPV infection were determined. The relative stable or upregulated expression level of BmAtlatin‐n, BmTHY, Bmseroin1, and Bmseroin2 in YeA resistant strain following BmNPV infection indicated their antiviral role in vivo, compared with susceptible YeB strain. Moreover, the significant downregulation of BmFerHCH, BmNHR96, and BmSINAL10 in both strains following BmNPV infection revealed their role in beneficial virus infection, as well as the upregulation of BmFerHCH in the midgut and BmSINAL10 in the hemolymph. Highlights: 1. Seven candidate genes showed significate immune response to BmNPV infection. 2. BmAtlatin‐n, BmTHY, Bmseroin1, and Bmseroin2 were resistant to BmNPV infection. 3. BmFerHCH, BmNHR96, and BmSINAL10 were susceptible to BmNPV infection.