In vitro biocompatibility of a sandblasted, acid-etched HA composite coating on ultrafine-grained titanium. Issue 11 (3rd February 2021)

• Record Type: Journal Article
• Authors: Chi, Yanxia; An, Sipeng; Xu, Yunpeng; Liu, Mingda; Zhang, Jie
• Is Part Of: RSC advances. Volume 11:Issue 11(2021)
• Page Start: 6124
• Page End: 6130
• Publication Date: 2021-02-03
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra10146j
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A sandblasted, acid-etched hydroxyapatite (SLA-HA) composite coating on ultrafine-grained titanium was synthesized by sandblasting, acid etching and electrophoresis deposition. Abstract : A sandblasted, acid-etching hydroxyapatite (SLA-HA) composite coating on ultrafine-grained titanium was synthesized by the sandblasting, acid etching and electrophoresis deposition. Mouse osteoblasts (MC3T3-E1) were cultured in vitro and inoculated on the SLA-HA composite coating of the ultrafine-grained titanium. Using ultrafine-grained titanium with SLA coating as the control group, the adhesion and proliferation of the osteoblasts were analyzed using the CCK-8 assay. The number and morphology of the cells were observed using a laser confocal microscope. Cells toxicity of the cytotoxicity to osteoblasts was studied by culturing them in an immersion solution of the SLA-HA composite coating. The hemolysis properties of the obtained material were assessed using fresh rabbit blood. Ultrafine-grained titanium with the SLA-HA composite coating was found to have no significant toxicity to osteoblasts, as well as good blood compatibility, playing a positive role in the adhesion of osteoblasts and promoting their proliferation and differentiation.