Simultaneous Estimation and Validation of Atorvastatin Calcium and Aspirin in Combined Capsule Dosage Form by RP HPLC Method. Issue 3 (2012)

• Record Type: Journal Article
• Authors: Suma, B. V.; Kannan, K.; Madhavan, V.; Nayar, Chandini R.
• Is Part Of: E-Journal of Chemistry. Volume 9:Issue 3(2012)
• Page Start: 1449
• Page End: 1456
• Publication Date: 2012
• DOI: 10.1155/2012/789538
• Languages: English
• ISSNs: 0973-4945

Abstract

Abstract : A new simple, specific, precise and accurate revere phase liquid chromatography method has been developed for estimation of atorvastatin calcium (AST) and ASPIRIN (ASP) simultaneously in a combined capsule dosage forms. The chromatographic separation was achieved on a 5 – micron C 18 column (250x 4.6mm) using a mobile phase consisting of a mixture of Acetonitrile: Ammonium Acetate buffer 0.02M (68:32) pH 4.5. The flow rate was maintained at 0.8 ml/min. The detection of the constituents was done using UV detector at 245 nm for AST and ASP. The retention time of AST and ASP were found be 4.5915 ± 0.0031 min and 3.282 ±0.0024 min respectively. The developed method was validated for accuracy, linearity, precision, limit of detection (LOD) and limit of quantification (LOQ) and robustness as per the ICH guidelines.