Development, Validation and Statistical Correlation of RP–LC Methods for Determination of Atazanavir Sulfate in Capsule Dosage Form. Issue 4 (2012)

• Record Type: Journal Article
• Authors: Behera, A.; Sankar, D. G.; Moitra, S. K.; Si, S. C.
• Is Part Of: E-Journal of Chemistry. Volume 9:Issue 4(2012)
• Page Start: 1778
• Page End: 1787
• Publication Date: 2012
• DOI: 10.1155/2012/402970
• Languages: English
• ISSNs: 0973-4945

Abstract

Abstract : To study the effective therapeutic bioavailability of Atazanavir Sulfate (ATV), administered singly or in combination with Ritonavir, a cost effective and rapid method is required. In order to assess an in-depth study, it is primarily thought prudent to develop an effective analytical method for estimation of ATV in marketed dosage forms. The present work is to develop a simple and precise analytical method for in depth evaluation of therapeutic efficacy of ATV. The novelty of the method shows linearity in the concentration range of 10-100 µg/mL at two wavelengths, i.e. 254 nm and 284 nm respectively. The chromatographic system consists of HiQSil C18HS column; an isocratic mobile phase consisted of methanol and tetrahydrofuran (95:5 v/v). The developed method is validated according to ICH guidelines in capsule dosage form. Validation of the developed method shows good result in range, linearity, accuracy and precision. Student's t –test was used to correlate the two methods and applied to raw materials and capsule dosage form.