Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS. Issue 4 (2011)

• Record Type: Journal Article
• Authors: Rajput, Mithlesh; Dahiya, Meenakshi; Kumari, Premlata; Kalra, Kamini; Aggarwal, Manjeet; Khandal, R. K.
• Is Part Of: E-Journal of Chemistry. Volume 8:Issue 4(2011)
• Page Start: 1770
• Page End: 1783
• Publication Date: 2011
• Subjects: Voglibose -- LC-MS/MS -- α-Glucosidase inhibitor -- Multiple reaction monitoring
• DOI: 10.1155/2011/531762
• Languages: English
• ISSNs: 0973-4945

Abstract

Abstract : Voglibose is a potent α glucosidase inhibitor, used for the treatment of diabetes mellitus. For quantitative determination of voglibose in pharmaceutical formulations of low doses, simple, sensitive, accurate and precise LC-MS/MS method using electrospray ionization in positive mode was developed and validated. The method was found linear in the concentration range of 25.0-1200 ηg/mL with a correlation coefficient of 0.9998. The limit of detection (LOD) of the method was found to be 1.5 ηg/mL and limit of quantitation (LOQ) was achieved at 3.0 ηg/mL. The recoveries of voglibose from spiked samples at different concentration levels were found in the range of 98-102%. The proposed method was found suitable for quantitation of voglibose and for the determination of uniformity of content of the dosage units of the tablet formulations.