H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine. Issue 4 (2011)

• Record Type: Journal Article
• Authors: Kaur, Amandeep; Gupta, Usha
• Is Part Of: E-Journal of Chemistry. Volume 8:Issue 4(2011)
• Page Start: 1979
• Page End: 1985
• Publication Date: 2011
• Subjects: HPSAM -- Eosin -- Erythrosine -- Simultaneous Determination and Fe(III)
• DOI: 10.1155/2011/210259
• Languages: English
• ISSNs: 0973-4945

Abstract

Abstract : A new, simple, sensitive and selective H -point standard addition method (HPSAM) has been developed for resolving binary mixture of food colorants eosin and erythrosine, which show overlapped spectra. The method is based on the complexation of food dyes eosin and erythrosine with Fe(III) complexing reagent at pH 5.5 and solubilizing complexes in triton x-100 micellar media. Absorbances at the two pairs of wavelengths, 540 and 550 nm (when eosin acts as analyte) or 518 and 542 nm (when erythrosine act as analyte) were monitored. This method has satisfactorily been applied for the determination of eosin and erythrosine dyes in synthetic mixtures and commercial products.