Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic Solutions. Issue 3 (2012)

• Record Type: Journal Article
• Authors: Narendra, A.; Deepika, D.; Annapurna, M. Mathrusri
• Is Part Of: E-Journal of Chemistry. Volume 9:Issue 3(2012)
• Page Start: 1238
• Page End: 1243
• Publication Date: 2012
• DOI: 10.1155/2012/258261
• Languages: English
• ISSNs: 0973-4945

Abstract

Abstract : A reverse phase HPLC method is described for the determination of Dorzolamide in eye drops. Chromatography was carried on an Inertsil ODS 3V column using Acetonitrile : (0.02M) 1, Octane Sulphonic acid buffer (pH 3.5) (36:64 v/v) on isocratic mode at a flow rate of 1 mL/min with UV detection at 254 nm. The detector response was linear in the concentration range 4-720 µg/mL. The limit of detection and limit of quantification are found to be 0.7041 and 2.3483 µg/mL respectively. The method was validated as per the ICH guidelines. The proposed method is rapid, accurate and precise and can be applied for the routine analysis of dorzolamide in ophthalimic solutions.