T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?. (8th November 2020)

• Record Type: Journal Article
• Authors: Lauruschkat, Chris D; Page, Lukas; Etter, Sonja; Weis, Philipp; Gamon, Florian; Kraus, Sabrina; Einsele, Hermann; Wurster, Sebastian; Loeffler, Juergen
• Is Part Of: Open forum infectious diseases. Volume 8:Number 1(2021)
• Publication Date: 2020-11-08
• Subjects: immunoassay -- biomarker -- aspergillosis -- cytomegalovirus -- T cells -- cytokines -- flow cytometry -- ELISPOT; Communicable diseases -- Periodicals; Medical microbiology -- Periodicals; Infection -- Periodicals; 616.9
• DOI: 10.1093/ofid/ofaa547
• Languages: English
• ISSNs: 2328-8957

Abstract

Abstract: We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus -reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood–based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.