A Broccoli aptamer chimera yields a fluorescent K+ sensor spanning physiological concentrations. Issue 11 (12th January 2021)

• Record Type: Journal Article
• Authors: Savage, Jonathan C.; Shinde, Pushkar; Yao, Yizhou; Davare, Monika A.; Shinde, Ujwal
• Is Part Of: Chemical communications. Volume 57:Issue 11(2021)
• Page Start: 1344
• Page End: 1347
• Publication Date: 2021-01-12
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc07042d
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We engineered a fluorescent mixed chemistry RNA chimera using 2′fluorinated pyrimidine analogues that detect K + spanning 0.1–100 mM. Abstract : The RNA aptamer Broccoli accepts 2′fluorinated (2′F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K + >30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K + concentrations, yielding an effective RNA-based fluorescent K + sensor.