Connecting the dots on vertical transmission of SARS-CoV-2 using protein-protein interaction network analysis – Potential roles of placental ACE2 and ENDOU. (15th January 2021)

• Record Type: Journal Article
• Authors: Jing, Hongwu; Ackerman, William E.; Zhao, Guomao; El Helou, Yara; Buhimschi, Catalin S.; Buhimschi, Irina A.
• Is Part Of: Placenta. Volume 104(2021)
• Page Start: 16
• Page End: 19
• Publication Date: 2021-01-15
• Subjects: Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2020.11.001
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: We conducted a protein-protein interaction (PPI) network study searching for proteins relevant to pregnancy-associated COVID-19 in pregnancy complicated with severe preeclampsia (sPE) and intra-amniotic infection and/or inflammation (Triple-I). PPI networks from sPE and Triple-I were intersected with the PPI network from coronavirus infection. Common proteins included the SARS-CoV-2 entry receptor ACE2 and ENDOU, a placental endoribonuclease homologous to Nsp15, a protein produced by the virus to escape host immunity. Remarkably, placental ENDOU mRNA expression far exceeded that of ACE2. Immunohistochemistry confirmed ENDOU localization at the hemochorial maternal-fetal interface. Investigation of ENDOU's relevance to vertical transmission of SARS-CoV-2 is further warranted. Highlights: A protein-protein interaction (PPI) network study for discovering proteins relevant to pregnancy-associated COVID-19. Placental Endoribonuclease, Poly(U) Specific (ENDOU, PP11), is homologous to Nsp15, an enzyme produced by coronavirus. Placental ENDOU mRNA expression far exceeded that of ACE2, the known cellular entry receptor for SARS-CoV-2. Immunohistochemistry confirmed ENDOU localization at the maternal-fetal interface. Investigation of ENDOU's relevance to vertical SARS-CoV-2 transmission is warranted.