Oxidative stress in the cyanobacterium Microcystis aeruginosa PCC 7813: Comparison of different analytical cell stress detection assays. (April 2021)

• Record Type: Journal Article
• Authors: Menezes, Indira; Maxwell-McQueeney, Declan; Capelo-Neto, José; Pestana, Carlos J.; Edwards, Christine; Lawton, Linda A.
• Is Part Of: Chemosphere. Volume 269(2021)
• Publication Date: 2021-04
• Subjects: Cyanobacteria -- Fluorescence -- Microcystin -- Water treatment -- Hydrogen peroxide; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.128766
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Cyanobacterial blooms are observed when high cell densities occur and are often dangerous to human and animal health due to the presence of cyanotoxins. Conventional drinking water treatment technology struggles to efficiently remove cyanobacterial cells and their metabolites during blooms, increasing costs and decreasing water quality. Although field applications of hydrogen peroxide have been shown to successfully suppress cyanobacterial growth, a rapid and accurate measure of the effect of oxidative stress on cyanobacterial cells is required. In the current study, H2 O2 (5 and 20 mg L −1 ) was used to induce oxidative stress in Microcystis aeruginosa PCC 7813. Cell density, quantum yield of photosystem II, minimal fluorescence and microcystin (MC-LR, -LY, -LW, -LF) concentrations were compared when evaluating M. aeruginosa cellular stress. Chlorophyll content (determined by minimal fluorescence) decreased by 10% after 48 h while cell density was reduced by 97% after 24 h in samples treated with 20 mg L −1 H2 O2 . Photosystem II quantum yield (photosynthetic activity) indicated cyanobacteria cell stress within 6 h, which was considerably faster than the other methods. Intracellular microcystins (MC-LR, -LY, -LW and -LF) were reduced by at least 96% after 24 h of H2 O2 treatment. No increase in extracellular microcystin concentration was detected, which suggests that the intracellular microcystins released into the surrounding water were completely removed by the hydrogen peroxide. Thus, photosynthetic activity was deemed the most suitable and rapid method for oxidative cell stress detection in cyanobacteria, however, an approach using combined methods is recomended for efficient water treatment management. Graphical abstract: Image 1 Highlights: Time lag observed between cell stress occurring and its detection by most methods. Photosynthetic activity analysis was the fastest method for cell stress detection. Advantages and drawbacks of five differrent cell stress detection assays elucidated.