Rib histomorphometry: A reliability and validation study with a critical review of histological techniques for forensic age estimation. (March 2021)

• Record Type: Journal Article
• Authors: García-Donas, Julieta G.; Bonicelli, Andrea; Scholl, Ashely Rose; Lill, Caroline; Paine, Robert R.; Kranioti, Elena F.
• Is Part Of: Legal medicine. Volume 49(2021)
• Publication Date: 2021-03
• Subjects: Age estimation -- Validation study -- Ribs -- Histomorphometry -- Mediterranean populations; Medical jurisprudence -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Medical jurisprudence; Periodicals; 614.1
• DOI: 10.1016/j.legalmed.2020.101827
• Languages: English
• ISSNs: 1344-6223

Abstract

Highlights: Age estimation by bone histomorphometry is often used on fragmented human remains. Histomorphometry aging methods must be validated if they are to be used forensically. Methods reliability, accuracy and bias are tested on two Mediterranean samples. Age estimation errors might be related to intrinsic factors and methodological issues. Abstract: Fragmented human remains present a challenge for forensic experts as they attempt to identify individuals using standard forensic methods. Several histological age estimation techniques have been developed during the last fifty years to aid in this process. However, very few validation studies have been conducted in order to test their accuracy and bias, and thus, validation assessment is required as we employ them while testifying in court. Histological variables are assessed from rib thin sections from two Mediterranean samples; Cretans (N = 41) and Greek-Cypriots (N = 47). Intra and inter-observer errors are assessed through TEM analysis and Intra-class Correlation Coefficient by testing observers with different levels of experience as they collected data on osteon counts and area measurements. The relation between the variables and age is determined using correlation coefficients. Histomorphometric data are applied to four widely used age estimation formulas assessing the performance of the methods for the entire sample. Inaccuracy and bias are calculated with age estimations and known age tested for significance and proportional bias assessed. Overall, histological parameters presented acceptable intra- and inter-observer errors. All variables exhibited statistically significant correlation with age (P < 0.01). For three of the techniques, data showed a systematic underestimation of age with an increase in inaccuracy in older individuals. One of the age estimation formulas produced overestimation of young individuals yet, it more accurately estimated the age of older individuals. This validation study explores inter-population variation in bone remodeling dynamics and presents a critical evaluation on methodological issues that can affect the performance of existing histological techniques.