Behavioral thermoregulation in the fasted C57BL/6 mouse. (February 2021)

• Record Type: Journal Article
• Authors: Craig, Molly C.; Silva, Larissa O.; Swoap, Steven J.
• Is Part Of: Journal of thermal biology. Volume 96(2021)
• Publication Date: 2021-02
• Subjects: Metabolic rate -- Torpor -- Preferred temperature -- Core temperature -- Behavior; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2020.102821
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: Under relatively cool ambient temperatures and a caloric deficit, mice will undergo daily torpor – a short-term regulated reduction in metabolic rate with a concomitant drop in body temperature. Mice can alternatively achieve metabolic savings by utilizing behavioral changes, such as seeking a warmer environment. However, there is a lack of knowledge about the behavioral interaction between torpor utilization and thermotaxis. That is, if a fasted mouse is faced with a choice between a warm environment not conducive for torpor, and a cool environment that will induce torpor, which scenario will the fasting mouse choose? Here, the temperature preferences of fasted mice were studied using a temperature gradient device that allows a mouse to freely move along a gradient of temperatures. C57BL/6 mice were implanted with temperature telemeters that recorded location, core temperature (Tb ), and activity concurrently over a 23-h period in the thermal gradient. When the gradient was on, mice preferred the warm end of the gradient when fed (71 ± 4% of the time) and even more so when fasted (84 ± 2%). When the gradient was on, the fasted minimum Tb was significantly higher (34.4 ± 0.3 °C) than when the gradient was off (27.7 ± 1.6 °C). Further, fasted mice lost significantly more weight when the gradient was off despite maintenance of a metabolically favorable lower minimum Tb in this condition. These results indicate that fasted mice not only prefer warm ambient temperatures when given the choice, but that it is also the pathway with more favorable metabolic outcomes in a period of reduced caloric intake. Highlights: Torpor is not an obligatory response for fasted mice when a warm environment is available on a thermally graded surface. Fasting further increases the preference for a warm environment by mice. During fasting, behavioral access to a warm surface attenuated body weight loss and minimized hypothermia.