Lipid and carotenoid production by the Rhodosporidium toruloides mutant in cane molasses. (April 2021)

• Record Type: Journal Article
• Authors: Zheng, Xuehai; Hu, Rongfei; Chen, Duo; Chen, Jiannan; He, Wenjin; Huang, Luqiang; Lin, Chentao; Chen, Huibin; Chen, Youqiang; Zhu, Jinmao; Qi, Feng; Xue, Ting
• Is Part Of: Bioresource technology. Volume 326(2021)
• Publication Date: 2021-04
• Subjects: Rhodosporidium toruloides -- Cane molasses -- Lipid -- Torularhodin -- Torulene -- β-carotene; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.124816
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Cane molasses was used for the lipid and carotenoids accumulation in R. toruloides. The production of torularhodin from MT was 14.68-fold higher than those of the WT. A high quality reference genome of R. toruloides M18 was produced. Transcriptome revealed hub genes for lipid and carotenoids accumulation. Abstract: Cane molasses is beneficial for lipid and carotenoid production in microalgae. We made a survey for the lipid and carotenoid production profile of R. toruloides M18 (MT) with various concentrations of molasses under nitrogen-deficited conditions. The production of α-linolenate and torularhodin from MT were 1.22– and 14.68-fold higher than those of the wild-type strain. We observed that molasses at concentrations of 35 g/L and 70 g/L represented a cheap and environmentally friendly strategy for producing lipids and carotenoids. Transcriptome and WGCNA analysis demonstrated that the genes relevant to the lipid and carotenoid production, including MYB, bHLH, Δ-4 desaturase, Δ-12 desaturase and FA2H, were significantly highly expressed. The results indicated that molasses could represent an inexpensive means for achieving high lipids and carotenoids production in R. toruloides .