A pilot study to investigate the histomorphometric changes of murine maxillary bone around the site of mini-screw insertion in regenerated bone induced by anabolic reagents. (23rd March 2020)

• Record Type: Journal Article
• Authors: Keo, Preksa; Matsumoto, Yoshiro; Shimizu, Yasuhiro; Nagahiro, Shigeki; Ikeda, Masaomi; Aoki, Kazuhiro; Ono, Takashi
• Is Part Of: European journal of orthodontics. Volume 43:Number 1(2021)
• Page Start: 86
• Page End: 93
• Publication Date: 2020-03-23
• Subjects: Orthodontics -- Periodicals; 617.643
• DOI: 10.1093/ejo/cjaa018
• Languages: English
• ISSNs: 0141-5387

Abstract

Summary: Objective: The objective of this study was to investigate the histomorphometric changes around the site of mini-screw insertion in the regenerated bone which was induced by an anabolic-injection method using both anabolic peptide and bone morphogenetic protein 2 (BMP-2). Methods: Twenty-seven eight-week-old C57BL/6J male mice were used. Some mice received submucosal co-injections of anabolic peptide and BMP-2 just in front of the maxillary first molar. Screw insertion was then performed 4 weeks after injection. All mice underwent a weekly in vivo micro-focal X-ray computed tomography (µCT) analysis before being sacrificed at week 8. The bone formation activity was evaluated using fluorescent labelling in the undecalcified sections. The analyses, including screw insertion, were performed in the frontal plane, in front of the site of screw insertion. Results: Reconstructed µCT images revealed that the co-injection of anabolic reagents could lead to a gradual increase in the bone mineral density (BMD) of the injection-induced thickened bone by week 8. Both radiological and histomorphometric analyses indicated that screw insertion did not have any deleterious effects on either the BMD or the bone formation activity of the induced bone. Furthermore, the injection of anabolic reagents also led to an increase in the BMD of the underlying maxillary bone at the injection site. Conclusion: Our histomorphometric analyses suggest that performing such anabolic injection to thicken bone could stimulate bone formation in the basal bone as well as in the induced bone. Similar augmentation of bone formation could be obtained even after subsequent screw insertion at the site of the induced bone.