Enhancing the Nucleolytic Resistance and Bioactivity of Functional Nucleic Acids by Diverse Nanostructures through in Situ Polymerization‐Induced Self‐assembly. (16th December 2020)

• Record Type: Journal Article
• Authors: Yang, Lu; Liang, Mingwei; Cui, Cheng; Li, Xiaowei; Li, Long; Pan, Xiaoshu; Yazd, Hoda Safari; Hong, Min; Lu, Jianrong; Cao, Y. Charles; Tan, Weihong
• Is Part Of: Chembiochem. Volume 22:Number 4(2021)
• Page Start: 754
• Page End: 759
• Publication Date: 2020-12-16
• Subjects: aptamers -- nucleic acids -- nanostructures -- polymerization -- self-assembly; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202000712
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Functional nucleic acids (FNAs) are garnering tremendous interest owing to their high modularity and unique bioactivity. Three‐dimensional FNAs have been developed to overcome the issues of nuclease degradation and limited cell uptake. We have developed a new facile approach to the synthesis of multiple three‐dimensional FNA nanostructures by harnessing photo‐polymerization‐induced self‐assembly. Sgc8 aptamer and CpG oligonucleotide were modified as macro chain‐transfer reagents to mediate in situ polymerization and self‐assembly. Diverse structures, including micelles, rods, and short worms, afford these two FNAs afford these two FNAs with higher nuclease resistance in serum serum, greater cellular uptake efficiency, and increased bioactivity. Abstract : Micelles, rods, and worms : Photo‐PISA was used to develop a new approach to FNA‐polymer nanoparticles that exhibit a high‐density display of FNA strands. The 3D structures of our two FNAs conferred higher nuclease resistance, greater cellular uptake efficiency, and increased bioactivity. We envision that this strategy could be expanded to other FNAs to solve critical issues.