Fluorescence Imaging of Mammalian Cells with Cationic Conjugated Polyelectrolytes. Issue 2 (19th October 2020)

• Record Type: Journal Article
• Authors: Wang, Shanshan; Jagadesan, Pradeepkumar; Sun, Han; Hu, Rong; Li, Zhiliang; Huang, Yun; Liu, Libing; Wang, Shu; Younus, Muhammad; Schanze, Kirk S.
• Is Part Of: ChemPhotoChem. Volume 5:Issue 2(2021)
• Page Start: 123
• Page End: 130
• Publication Date: 2020-10-19
• Subjects: Aggregation -- confocal laser scanning microscopy -- conjugated polyelectrolytes -- bioimaging -- fluorescence imaging; Photochemistry -- Periodicals; Periodicals; Electronic journals; 541.35
• DOI: 10.1002/cptc.202000192
• Languages: English
• ISSNs: 2367-0932

Abstract

Abstract: Fluorescence imaging using poly(phenylene ethynylene) (PPE) based conjugated polyelectrolytes (CPEs) has garnered considerable attention in the recent past due to their remarkable photophysical properties. In this report, we studied the fluorescence imaging properties of a set of six PPE‐based CPEs appended with side chains varying in structure and number of cationic charges. The photophysical and aggregation features of the CPEs depend on the overall charge density on the polymer in water. The cytotoxicity of the CPEs was evaluated against MCF‐7 mammalian cells, which revealed that polyelectrolytes with less cationic charge are less cytotoxic and vice versa . Confocal laser scanning microscopy (CLSM) studies showed that all the CPEs could efficiently penetrate into the MCF‐7 cells after 10 h of incubation. The transit of CPEs into the lysosomes was confirmed by comparison with the uptake of LysoTracker Red DND‐99 dye, which also revealed that the polyelectrolytes are highly photostable. We also demonstrated that the imidazolium‐functionalized CPEs could selectively stain bacteria over mammalian cells, suggesting the importance of CPEs for clinical applications. Abstract : On target : The fluorescence imaging properties of a series of poly(phenylene ethynylene) (PPE) based conjugated polyelectrolytes (CPEs) appended with side chains varying in structure and number of cationic charges were investigated. The cytotoxicity of the CPEs was evaluated against MCF‐7 mammalian cells and confocal laser scanning microscopy was used to demonstrate that all CPEs could efficiently penetrate into the cells after 10 h of incubation.