Instant polarized light microscopy for imaging collagen microarchitecture and dynamics. Issue 2 (5th November 2020)

• Record Type: Journal Article
• Authors: Yang, Bin; Lee, Po‐Yi; Hua, Yi; Brazile, Bryn; Waxman, Susannah; Ji, Fengting; Zhu, Ziyi; Sigal, Ian A.
• Is Part Of: Journal of biophotonics. Volume 14:Issue 2(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-11-05
• Subjects: biomechanics -- collagen -- deformation -- polarized light microscopy; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.202000326
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Collagen fibers are a primary load‐bearing component of connective tissues and are therefore central to tissue biomechanics and pathophysiology. Understanding collagen architecture and behavior under dynamic loading requires a quantitative imaging technique with simultaneously high spatial and temporal resolutions. Suitable techniques are thus rare and often inaccessible. In this study, we present instant polarized light microscopy (IPOL), in which a single snapshot image encodes information on fiber orientation and retardance, thus fulfilling the requirement. We utilized both simulation and experimental data from collagenous tissues of chicken tendon, sheep eye, and porcine heart to evaluate the effectiveness of IPOL as a quantitative imaging technique. We demonstrate that IPOL allows quantitative characterization of micron‐scale collagen fiber architecture at full camera frame rates (156 frames/second herein). Abstract : Collagen fibers are the primary load‐bearing component of connective tissues and central to their biomechanics and pathophysiology. Understanding collagen architecture and behavior under dynamic loading requires a quantitative imaging technique with simultaneously high spatial and temporal resolutions. Instant polarized light microscopy (IPOL) was developed to meet these needs, by allowing quantitative snapshot imaging of collagen, providing information on fiber architecture, orientation and retardance.