A Hierarchical Janus Nanofibrous Membrane Combining Direct Osteogenesis and Osteoimmunomodulatory Functions for Advanced Bone Regeneration. (20th November 2020)

• Record Type: Journal Article
• Authors: Wang, Qian; Feng, Yunbo; He, Min; Zhao, Weifeng; Qiu, Li; Zhao, Changsheng
• Is Part Of: Advanced functional materials. Volume 31:Number 8(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-11-20
• Subjects: antibacterial -- electrospinning -- guided bone regeneration -- osteogenesis -- osteoimmunomodulation; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202008906
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: An ideal guided bone regeneration membrane (GBRM) is expected not only to perform barrier function, but also to enhance osteogenesis and resist bacteria infection. However, currently available membranes have limited bioactivities. To address this challenge, a Janus GBRM (JGM) is designed and fabricated by sequential fractional electrospinning here. The random gelatin fibers loaded with hydroxyapatite (HAP) are designed as the inner face to promote the osteoblasts' adhesion, proliferation, and osteogenic differentiation, meanwhile the aligned poly(caprolactone) (PCL) nanofibers loaded with poly(methacryloxyethyltrimethyl ammonium chloride‐ co ‐2‐Aminoethyl 2‐methylacrylate hydrochloride) (P(DMC‐AMA)) are designed as the outer layer to resist epithelia invasion and bacterial infection. In vitro assays reveal that the inner face displays enhanced osteogenic effects, meanwhile the outer surface can regulate the epithelia to spread along the aligned direction and kill the contacted bacteria. Interestingly, the outer face can induce macrophages to polarize toward the M2 phenotype, thus manipulating a favorable osteoimmune environment. These results suggest that the JGM simultaneously meets the critical requirements of barrier, osteogenic, antibacterial, and osteoimmunomodulatory functions. Consequently, the JGM shows better in vivo bone tissue regeneration performance than the commercial Bio‐Gide membrane. This work provides a novel platform to design multi‐functional membranes/scaffolds, displaying great potential applications in tissue engineering. Abstract : Herein, a Janus guided bone regeneration membrane (JGM) with multiple functions is fabricated. The inner face functions to promote osteogenic differentiation of osteoblasts directly, meanwhile the outer face functions to provide a bone‐encouraging immune microenvironment for bone repair. Additionally, the outer face protects the bone defect from endotheliocyte incursion and bacterial infection. In vivo experiments confirm the superior features of the JGM for bone regeneration.