Functional requirement of the Arabidopsis importin‐α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1. (25th December 2020)

• Record Type: Journal Article
• Authors: Lüdke, Daniel; Roth, Charlotte; Kamrad, Sieglinde A.; Messerschmidt, Jana; Hartken, Denise; Appel, Jonas; Hörnich, Bojan F.; Yan, Qiqi; Kusch, Stefan; Klenke, Melanie; Gunkel, Annette; Wirthmueller, Lennart; Wiermer, Marcel
• Is Part Of: Plant journal. Volume 105:Number 4(2021)
• Page Start: 994
• Page End: 1009
• Publication Date: 2020-12-25
• Subjects: Arabidopsis -- IMPORTIN‐α -- nucleotide‐binding leucine‐rich repeat (NLR) -- nuclear import -- plant immunity -- SNC1; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.15082
• Languages: English
• ISSNs: 0960-7412

Abstract

SUMMARY: IMPORTIN‐α3/MOS6 (MODIFIER OF SNC1, 6) is one of nine importin‐α isoforms in Arabidopsis that recruit nuclear localization signal‐containing cargo proteins to the nuclear import machinery. IMP‐α3 / MOS6 is required genetically for full autoimmunity of the nucleotide‐binding leucine‐rich repeat immune receptor mutant snc1 ( suppressor of npr1‐1, constitutive 1 ) and MOS6 also contributes to basal disease resistance. Here, we investigated the contribution of the other importin‐ α genes to both types of immune responses, and we analyzed potential interactions of all importin‐α isoforms with SNC1. By using reverse‐genetic analyses in Arabidopsis and protein−protein interaction assays in Nicotiana benthamiana, we provide evidence that among the nine α‐importins in Arabidopsis, IMP‐α3/MOS6 is the main nuclear transport receptor of SNC1, and that IMP‐α3 / MOS6 is required selectively for autoimmunity of snc1 and basal resistance to mildly virulent Pseudomonas syringae in Arabidopsis . Significance Statement: Specific requirement for the Arabidopsis α‐importin MOS6 in snc1 ‐mediated autoimmunity is explained by selective formation of MOS6‐SNC1 nuclear import complexes.