Determination of Quinic Acids in Helichrysum arenarium (L.) Moench by Ultrafiltration Affinity and Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Time-of-Flight Mass Spectrometry (UF-UPLC-Q-TOF-MS). (24th March 2021)

• Record Type: Journal Article
• Authors: Yang, Jinxiang; Qi, Yunyun; Li, Hongye; Jiang, Min; Zhu, Yanyan; Xue, Rui; Yu, Lili; Chen, Wen; Bo Han,
• Is Part Of: Analytical letters. Volume 54:Number 5(2021)
• Page Start: 772
• Page End: 789
• Publication Date: 2021-03-24
• Subjects: Helichrysum arenarium (L.) Moench -- molecular docking -- pancreatic lipase inhibitors -- quinic acids -- UF-UPLC-Q-TOF-MS; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2020.1781876
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: Helichrysum arenarium (L.) Moench, a traditional herbal medicine distributed in Europe and Central Asia, has been used to lower blood lipids and treat obesity. However, the active compounds of H. arenarium, and the potential mechanism by which they reduce obesity remain unclear. The primary objective of this study was to identify the anti-obesity compounds in H. arenarium extracts. Based on the compound library of Helichrysum species, 55 compounds, including six with anti-obesity activity, have been identified by ultrafiltration affinity and ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UF-UPLC-Q-TOF-MS). Interestingly, all of the detected active compounds were derivatives of caffeoylquinic acid. Among them, isochlorogenic acid B showed the most potent inhibitory activity toward lipase, with an IC50 value of 12.63 ± 2.99 μg/mL. The potential mechanisms of the six active compounds for the treatment of obesity were explored. This work presents an efficient method for rapid screening lipase inhibitors from complex natural products, which can be easily adapted to other therapeutically relevant enzymatic systems. Additionally, the results revealed the chemical basis and mechanism of the anti-obesity properties of H. arenarium.