G-quadruplex binding protein Rif1, a key regulator of replication timing. (10th November 2020)

• Record Type: Journal Article
• Authors: Alavi, Sana; Ghadiri, Hamed; Dabirmanesh, Bahareh; Moriyama, Kenji; Khajeh, Khosro; Masai, Hisao
• Is Part Of: Journal of biochemistry. Volume 169:Number 1(2021)
• Page Start: 1
• Page End: 14
• Publication Date: 2020-11-10
• Subjects: chromatin structure -- DNA replication -- G-quadruplexes -- DNA–protein interaction -- Rap1 interacting factor 1 (Rif1) -- replication timing; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Electronic journals; 572.05
• DOI: 10.1093/jb/mvaa128
• Languages: English
• ISSNs: 0021-924X

Abstract

Abstract: DNA replication is spatially and temporally regulated during S phase to execute efficient and coordinated duplication of entire genome. Various epigenomic mechanisms operate to regulate the timing and locations of replication. Among them, Rif1 plays a major role to shape the 'replication domains' that dictate which segments of the genome are replicated when and where in the nuclei. Rif1 achieves this task by generating higher-order chromatin architecture near nuclear membrane and by recruiting a protein phosphatase. Rif1 is a G4 binding protein, and G4 binding activity of Rif1 is essential for replication timing regulation in fission yeast. In this article, we first summarize strategies by which cells regulate their replication timing and then describe how Rif1 and its interaction with G4 contribute to regulation of chromatin architecture and replication timing. Graphical Abstract: