Transcriptome-targeted analysis of human peripheral blood-derived macrophages when cultured on biomaterial meshes. (17th February 2021)

• Record Type: Journal Article
• Authors: Mora-Navarro, Camilo; Ozpinar, Emily W; Sze, Daphne; Martin, David P; Freytes, Donald O
• Is Part Of: Biomedical materials. Volume 16:Number 2(2021)
• Publication Date: 2021-02-17
• Subjects: surgical meshes -- macrophage polarization -- biomaterials -- targeted transcriptome -- poly-4-hydroxybutyrate (P4HB) -- polypropylene; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/abdbdb
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: Surgical meshes are commonly used to repair defects and support soft tissues. Macrophages (M φ s) are critical cells in the wound healing process and are involved in the host response upon foreign biomaterials. There are various commercially available permanent and absorbable meshes used by surgeons for surgical interventions. Polypropylene (PP) meshes represent a permanent biomaterial that can elicit both inflammatory and anti-inflammatory responses. In contrast, poly-4-hydroxybutyrate (P4HB) based meshes are absorbable and linked to positive clinical outcomes but have a poorly characterized immune response. This study evaluated the in vitro targeted transcriptomic response of human M φ s seeded for 48 h on PP and P4HB surgical meshes. The in vitro measured response from human M φ s cultured on P4HB exhibited inflammatory and anti-inflammatory gene expression profiles typically associated with wound healing, which aligns with in vivo animal studies from literature. The work herein provides in vitro evidence for the early transcriptomic targeted signature of human M φ s upon two commonly used surgical meshes. The findings suggest a transition from an inflammatory to a non-inflammatory phenotype by P4HB as well as an upregulation of genes annotated under the pathogen response pathway.