Molecular Diagnosis and Prevalence of Trypanosoma vivax (Trypanosomatida: Trypanosomatidae) in Buffaloes and Ectoparasites in the Brazilian Amazon Region. Issue 1 (22nd July 2020)

• Record Type: Journal Article
• Authors: Dyonisio, Gustavo Henrique Souza; Batista, Helder Ribeiro; da Silva, Ryan Emiliano; Azevedo, Roberta Carvalho de Freitas e; Costa, Jaciara de Oliveira Jorge; Manhães, Ingridi Braz de Oliveira; Tonhosolo, Renata; Gennari, Solange Maria; Minervino, Antonio Humberto Hamad; Marcili, Arlei
• Editors: Ginsberg, Howard
• Is Part Of: Journal of medical entomology. Volume 58:Issue 1(2021)
• Page Start: 403
• Page End: 407
• Publication Date: 2020-07-22
• Subjects: Trypanosomiasis -- tick -- lice -- Amazonia -- molecular diagnosis; Insects as carriers of disease -- Periodicals; 616.968
• DOI: 10.1093/jme/tjaa145
• Languages: English
• ISSNs: 0022-2585

Abstract

Abstract: Trypanosoma vivax Ziemann is a parasite that affects both wild and domestic ungulates and is transmitted mechanically via tabanids and other blood-sucking insects in the Americas. A total of 621 blood samples from water buffaloes ( Bubalus bubalis (Linnaeus) (Artiodactyla: Bovidae), and 184 ectoparasite samples ( Amblyomma cajennense (Fabricius) sensu stricto and Rhipicephalus ( Boophilus ) microplus (Canestrini) (Acari: Ixodidae), and Haematopinus tuberculatus (Burmeister) (Phthiraptera: Haematopinidae)) were obtained from 60 farms in the State of Pará, Brazilian Amazon. Twelve buffalo blood samples (1.89%) and 11 ectoparasites (6%) were positive for T. vivax based on the cathepsin L-like gene. All sequences were 99% similar to T. vivax from northeastern Brazil (EU753788) in amplified PCR assays on each of the hosts tested.