Photobacterium chitinilyticum sp. nov., a marine bacterium isolated from seawater at the bottom of the East China Sea. Issue 5 (12th March 2019)

• Record Type: Journal Article
• Authors: Wang, Xiaolei; Li, Yuying; Xue, Chun-Xu; Li, Bei; Zhou, Shun; Liu, Lijun; Zhang, Xiao-Hua
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 5(2019)
• Page Start: 1477
• Page End: 1483
• Publication Date: 2019-03-12
• Subjects: Photobacterium chitinilyticum -- bottom seawater -- East China Sea; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003343
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, facultative aerobic, motile by a polar flagellum, rod-shaped strain, designated BEI247 T, was isolated from seawater at the bottom of the East China Sea. Phylogenetic analysis of the 16S rRNA gene and whole genome data affiliated it with the genus Photobacterium . It was most closely related to Photobacterium alginatilyticum P03D4 T (97.36 % 16S rRNA gene similarity). Multi-locus sequence analysis (MLSA) revealed a distinct lineage with P. alginatilyticum P03D4 T as its closest relative. Strain BEI247 T was found to have lower than 86.0 % similarities to the type strains of its most closely related species in MLSA, less than 82.3 % using genome average nucleotide identities, and less than 25.3 % in DNA–DNA relatedness studies. Growth occurred at 10–37 °C (optimum, 24 °C), pH 5.0–8.0 (pH 7.0) and in the presence of 1–5 % (w/v) NaCl (3 %). The dominant fatty acids were summed feature 3 (C16 : 1 ω7 c and/or C16 : 1 ω6 c ) and C16 : 0 . The polar lipids of strain BEI247 T comprised phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, two phospholipids and one unknown lipid. The major respiratory quinone was ubiquinone-8 (Q-8). The DNA G+C content of strain BEI247 T was 46.45 mol%. On the basis of the polyphasic evidence, strain BEI247 T is proposed as representing a novel species of the genus Photobacterium, for which the name Photobacterium chitinilyticum sp. nov. is proposed. The type strain is BEI247 T (=JCM 32689 T =MCCC 1K03517 T =KCTC 62619 T ).