Major differences in the larval anatomy of the digestive and excretory systems of three Oestridae species revealed by micro‐CT. Issue 1 (8th September 2020)

• Record Type: Journal Article
• Authors: Martín‐Vega, D.; Clark, B.; Ferrer, L. M.; López‐Tamayo, S.; Panadero, R.; Cepeda‐Palacios, R.; Colwell, D. D.; Hall, M. J. R.
• Is Part Of: Medical and veterinary entomology. Volume 35:Issue 1(2021)
• Page Start: 106
• Page End: 120
• Publication Date: 2020-09-08
• Subjects: Cephenemyia stimulator -- Hypoderma actaeon -- Oestrus ovis -- alimentary canal -- foregut -- hindgut -- Malpighian tubules -- midgut -- organ volume -- salivary glands; Entomology -- Periodicals; Veterinary entomology -- Periodicals; Insects as carriers of disease -- Periodicals; 616.968
• DOI: 10.1111/mve.12476
• Languages: English
• ISSNs: 0269-283X

Abstract

Abstract: Oestrid flies (Diptera: Oestridae) do not feed during the adult stage, so they depend on an efficient assimilation and storage of nutrients during their parasitic larval stage. We describe the general morphology and provide volumetric data for the digestive and excretory organs of the three larval instars of the nasal bot fly Oestrus ovis L., using micro‐computed tomography. The size of the digestive and excretory organs greatly increased across larval instars. In all instars, the two salivary glands were remarkably large and formed a 'glandular band' by coming together, but without lumina uniting, at their posterior ends. The distal region of the anterior Malpighian tubules was greatly enlarged and full of highly radio‐opaque concretions. Moreover, the anatomy of O. ovis third‐instar larva was compared to that of two species of, respectively, similar and different feeding habits: Cephenemyia stimulator (Clark) and Hypoderma actaeon Brauer. Whereas the general morphology and arrangement of the digestive and excretory systems of C. stimulator was similar to that of O. ovis, some differences were observed in H. actaeon : a swollen anterior region of the midgut, salivary glands shorter and not forming a 'band' and anterior Malpighian tubules narrowly uniform throughout their entire length. Abstract : The anatomy of the digestive and excretory systems of the three larval instars of Oestrus ovis is described with the use of micro‐computed tomography. The anatomy of the digestive and excretory systems of the third‐instar larva of O. ovis is compared to that of Cephenemyia stimulator and Hypoderma actaeon . The morphology of the organs is similar in Oestrus ovis and Cephenemyia stimulator, whereas the morphology and arrangement of the midgut, salivary glands and Malpighian tubules differ in Hypoderma actaeon .