Site-specific polymerase incorporation of consecutive ligand-containing nucleotides for multiple metal-mediated base pairing. Issue 11 (13th January 2021)

• Record Type: Journal Article
• Authors: Nakama, Takahiro; Takezawa, Yusuke; Shionoya, Mitsuhiko
• Is Part Of: Chemical communications. Volume 57:Issue 11(2021)
• Page Start: 1392
• Page End: 1395
• Publication Date: 2021-01-13
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc07771b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : An enzymatic method has been developed for the synthesis of DNA oligomers containing consecutive unnatural ligand-type nucleotides forming multiple Cu II -mediated base pairs. Abstract : An enzymatic method has been developed for the synthesis of DNA oligomers containing consecutive artificial ligand-type nucleotides. Three hydroxypyridone ligand-containing nucleotides forming Cu II -mediated unnatural base pairs were continuously incorporated at a pre-specified position by a lesion-bypass Dpo4 polymerase. This enzymatic synthesis was applied to the development of a Cu II -responsive DNAzyme. Accordingly, this research will open new routes for the construction of metal-responsive DNA architectures that are manipulated by multiple metal-mediated base pairing.