Anti–Interleukin-10 Unleashes Transcriptional Response to Leishmanial Antigens in Visceral Leishmaniasis Patients. (2nd July 2020)

• Record Type: Journal Article
• Authors: Singh, Om Prakash; Syn, Genevieve; Nylén, Susanne; Engwerda, Christian; Sacks, David; Wilson, Mary E; Kumar, Rajiv; Chakravarty, Jaya; Sundar, Shyam; Blackwell, Jenefer M; Fakiola, Michaela
• Is Part Of: Journal of infectious diseases. Volume 223:Number 3(2021)
• Page Start: 517
• Page End: 521
• Publication Date: 2020-07-02
• Subjects: visceral leishmaniasis -- RNA expression -- whole-blood assays -- anti -- interleukin-10 -- soluble leishmania antigen -- cytokine storm; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiaa381
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Visceral leishmaniasis (VL; Leishmania donovani ) cases produce interferon-γ and tumor necrosis factor in response to soluble leishmanial antigen (SLA) in whole-blood assays. Using transcriptional profiling, we demonstrate the impact of interleukin-10 (IL-10), a cytokine implicated in VL, on this response. SLA stimulation identified 28 differentially expressed genes (DEGs), 17/28 in a single network with TNF as hub. SLA plus anti–IL-10 produced 454 DEGs, 292 in a single network with TNF, IFNG, NFKBIA, IL6, and IL1B as hubs in concert with a remarkable chemokine/cytokine storm. Our data demonstrate the singular effect of IL-10 as a potent immune modulator in VL.